PLANT TRANSFORMATION LABORATORY

MISSION STATEMENT

CROP SCIENCE, plant transformtaion laboratory

A major goal of the Plant Transformation Laboratory (PTL) is to serve as the local provider for incorporating the most advanced gene delivery and molecular analysis technologies into the plant science programs at North Carolina State University. Although current plant transformation is well established for numerous plant species, high throughput transformation and stable gene expression remain elusive. This is especially true in elite breeding stocks. When stable transformation is achieved, it is then necessary to analyze multiple independent transformation events for continued stability of the transgenic trait. Therefore, the PTL has the equipment and expertise to assist researchers in the necessary downstream analysis of post-transformation events as well.

The NCSU PTL will collaborate with researchers who seek to use plant transformation in their research programs by serving as a resource for obtaining and working with different transformation vectors and methodologies. As part of the collaboration, the PTL has the necessary experienced personal, equipment and reagents to work with transgenes, transformation and regeneration of plants, and the subsequent testing of individual transformants for transgene integrity and expression of the desired trait. In addition, as part of a basic research mission, the PTL will carry out resarch to develop new transformation technologies in order to remain at the cutting edge of plant biotechnology and to provide novel technologies that are free of Intellectual Property constraints.